Skip to main content
Figure 2 | Molecular Cancer

Figure 2

From: The chemopreventive retinoid 4HPR impairs prostate cancer cell migration and invasion by interfering with FAK/AKT/GSK3β pathway and β-catenin stability

Figure 2

4HPR inhibits FAK and AKT activity in prostate cancer cells, thus controlling their migratory potential and VEGF release. Western blot analyses show a remarkable decrease of FAK and AKT phosphorylation after 4 h of treatment with 4HPR in DU145 (A) and PC3 (B) cells. C). The specific PI3K/AKT inhibitors Wortmannin (Wort, 200 nM) and LY294002 (LY, 10 μM) significantly impair the chemotaxis of DU145 cells towards FB-CM similar to 4HPR (5 μM). Co-treatment with Wortmannin and 4HPR produces an even more pronounced effect. Similar results were obtained with PC3 cells. Experiments were done in triplicate and repeated thrice. Comparisons were made with control cells. Means ± SD are shown (***P < 0.001). D). Decreased AKT activity induced by 4HPR correlates with reduced VEGF secretion by DU145 and PC3 exposed for 16 h to the drug and analyzed by ELISA. Means ± SD are shown (*P < 0.05, **P < 0.01, ***P < 0.001).

Back to article page

Molecular Cancer

ISSN: 1476-4598