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Figure 5 | Molecular Cancer

Figure 5

From: The chemopreventive retinoid 4HPR impairs prostate cancer cell migration and invasion by interfering with FAK/AKT/GSK3β pathway and β-catenin stability

Figure 5

β-catenin levels control prostate cancer cell proliferation, migration and invasiveness. A). DU145 and PC3 cells permanently transfected with β-catenin shRNA sequences (Sh- βcat) had decreased levels of β-catenin as compared to non-target shRNA control vector (sh-NT) transfected cells (insets) and showed decreased cell proliferation as evaluated by the crystal violet assay. β-catenin silenced DU145 and PC3 cells had impaired ability to migrate toward FB-conditioned medium (B) and to invade through matrigel (C). D). Sh- βcat DU145 cells were transiently cotransfected with activated AKT (Myr.Akt) or empty vector (vector) and 24 hours after transfection assayed for their ability to migrate toward FB-conditioned medium or to invade through matrigel. Although AKT activation greatly nullify the effects of β-catenin silencing on cell migration and invasion, activation of Akt alone was not able to phosphorylate and inhibit the GSK3β pool involved in β-catenin up-regulation, thus β-catenin levels remained unaltered (right panel). All results are representative of three independent experiments (***P < 0.001).

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Molecular Cancer

ISSN: 1476-4598