Figure 4

Analysis of p53 mutant protein conformation. p53 mutant protein conformation was analysed in A) MDA-MB-468 cells containing mostly folded p53 R273H mutant and B) SK-BR-3 cells containing only unfolded p53 R175H mutant. Cells were treated for 9 hours with 10 μM cisplatin, 10 μM LA-12 and 4 μM 17-AAG. Conformational changes of p53 were determined using immunoprecipitation with p53 conformation-specific mouse monoclonal antibodies PAb1620 (detects folded conformation) and PAb240 (detects unfolded conformation). CM-1 rabbit polyclonal antibody was used for detection of p53 protein.