Skip to main content
Figure 5 | Molecular Cancer

Figure 5

From: The new platinum(IV) derivative LA-12 shows stronger inhibitory effect on Hsp90 function compared to cisplatin

Figure 5

Analysis of p53 mutant protein degradation. p53 mutant protein levels were analysed in A) MDA-MB-468 cells containing mostly folded p53 R273H mutant and B) SK-BR-3 cells containing unfolded p53 R175H mutant transfected with CHIP or CHIP-TPR protein by western blotting. Cells were treated for 9 hours with 10 μM cisplatin, 10 μM LA-12 and 4 μM 17-AAG. CM-1 rabbit polyclonal antibody and CHIP-11.1 mouse monoclonal antibody were used for detection of p53 and CHIP proteins. Actin was used as loading control.

Back to article page

Molecular Cancer

ISSN: 1476-4598