Figure 6From: The new platinum(IV) derivative LA-12 shows stronger inhibitory effect on Hsp90 function compared to cisplatinAnalysis of degradation of Hsp90 client proteins. Protein levels of p53, cyclin D1 (CD1) and estrogen receptor (ER) were determined in cells treated for 3, 6 and 9 hours with 10 μM cisplatin and 10 μM LA-12 by western blotting. CM-1 rabbit polyclonal antibody, SP1 rabbit monoclonal antibody and CD1 mouse monoclonal antibody was used for detection of p53, ER and CD1 proteins, respectively. Actin was used as loading control.Back to article page