Effects of PDEF expression in prostate cancer cells on cell morphology, FAK phosphorylation and attachment to specific substrates. A, Phalloidin staining of actin cytoskeleton in PDEF expressing and vector control cells. B, Decreased phophorylation of FAK in PDEF expressing cells growing in suspension culture. C, Representative photomicrographs from experiments testing attachment of PC3 cell expressing PDEF to either BSA or fibronectin or matrigel-treated plastic surface as described in Material and Methods; and quantitation of these data. Asterisks indicate significance levels of p < 0.05 with respect to controls.