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Figure 4 | Molecular Cancer

Figure 4

From: RETRACTED ARTICLE: Loss of PDEF, a prostate-derived Ets factor is associated with aggressive phenotype of prostate cancer: Regulation of MMP 9 by PDEF

Figure 4

Effect of PDEF on invasion through Matrigel Matrix and MMP9 mRNA expression, MMP9 promoter activity and MMP9 enzymatic activity of prostate cancer cells. A, Invasion of PC3 cells expressing PDEF or PDEF T50A through Transwell membranes as described in Methods. B, RT-PCR showing MMP9 mRNA (left panel) and MMP9 enzymatic activity using gelatin Zymography (Right panel) was performed to determine the level of active MMP9. C, MMP9 promoter activity as determined by Luciferase reporter assay. D, Invasion of PC3 cells through a basement membrane matrix after blocking MMP9 using antibodies or overexpressing PDEF. Asterisks indicate significance levels of p < 0.05 with respect to controls.

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