Effect of PDEF expression on cells grown in three dimensional cultures. A, representative images of three dimensional matrigel culture of PC3 cells with and without PDEF showing phenotypic reversal. Representative phase image obtained at various days in culture using 20X objective. B , Fluorescence microscopic images of PDEF PC3 cells and vector control cells after phalloidin and DAPI staining in 3 D cultures. C) Schematic depiction of the effects of PDEF expression in Prostate cancer cells.