Figure 14
From: Polycomb repressor complex 2 regulates HOXA9 and HOXA10, activating ID2 in NK/T-cell lines
Regulation of EZH2 expression via E2F/TFDP1. (A) Western blot analysis of selected NK/T-cell lines demonstrated the presence of E2F1 protein in all cells including LOUCY. (B) RT-PCR analysis of TFDP1 in selected NK/T-cell lines demonstrated reduced expression level in LOUCY. Expression of TEL served as positive control, NTC: no template control. (C) RQ-PCR analysis of LOUCY cells which were lentivirally transduced with an expression construct for TFDP1 demonstrated increased expression levels of EZH2 and decreased levels of HOXA10 and ID2 as compared to vector control cells.