Figure 1

Angiogenesis assay using Matrigel plugs in SCID mice. Matrigel containing different reagents was subcutaneously injected into SCID mice as described in materials and methods. (A, B) Matrigel was mixed with MDA-MB-231 breast cancer cells and isotype control or anti-NGF neutralizing antibodies (75 μg/ml); (C) Matrigel was mixed with proNGF (7.5 μg/ml), NGF (3.75 μg/ml), or VEGF (0.375 μg/ml). Angiogenesis was analyzed by quantification of hemoglobin (A, C) and microvessel density (B) as described in materials and methods. Five mice were used for each group and results are the mean of three independent experiments. Student's t-test, *p < 0.01 versus control.