Figure 2
From: Nerve growth factor promotes breast cancer angiogenesis by activating multiple pathways
Pleiotropic effects of NGF on HUVEC. (A) Growth assay. HUVEC cultured on standard culture plastic were treated with NGF (100 ng/ml), or VEGF (10 ng/ml) for 48 h. (B and C) Migration and invasion assay using Transwells. (D) Cord formation assay on matrigel-coated 24-well dishes. (E) Endothelial cell monolayer permeability assay. The kinetics of 70 kDa Dextran-FITC having passed through the monolayer of endothelial cells was determined by measurement of fluorescence in the lower chambers at different time points. A to D Student's t-test, *p < 0.01 versus control. E, ANOVA test, p < 0.05.