Figure 5

Involvement of NO synthase in NGF-stimulated invasion. (A) Western blot analysis of phospho NO Synthase (pNOS) upon NGF (100 ng/ml) treatment. Results were representative of two independent experiments. (B) Quantification of NO levels in HUVEC. Cells were pretreated with NO synthase inhibitor (L-NAME, 0.1 mM) for 30 min, then loaded with DAF-2DA for 20 min before treatment with NGF in the presence or absence of L-NAME for 2 h. (C) Illustration of fluorescence intensity, which represent the levels of NO in HUVEC under different conditions. (D) Invasion assay using Transwells. HUVEC were pretreated with L-NAME and then treated with NGF for 24 h. For both B and D, Student's t-test, *p < 0.01 versus control.