Figure 2

The ΔPS mutant of BMI1 is more stable than the wild type BMI1. (A).The half life of the ΔPS mutant of BMI1 and the wild type BMI1 was determined using CHX treatment (0-240 min) of MCF10A-BMI1WT and MCF10A-BMI1ΔPS cells as described above. (B). MCF10A-BMI1WT and MCF10A-BMI1ΔPS cells were treated with MG132 for different times points (as indicated) to determine its effect on the wild type and the ΔPS mutant of BMI1. Cells were harvested after each time point and analyzed for accumulation of the wild type or mutant BMI1 protein by western blot analysis. The accumulated proteins were quantified by densitometric analysis of signal present in respective lanes and by normalizing it to the individual α-tubulin signal. The densitometric analysis was done as described in Fig. 1B.