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Table 1 Analyses of Glioma Individual Cell Displacement

From: ROS accumulation and IGF-IR inhibition contribute to fenofibrate/PPARα -mediated inhibition of Glioma cell motility in vitro

Conditions\Parameters Total length of cell trajectory (μm) Average speed of cell movement (μm/min)a Length of final cell displacement (μm) Average rate of cell displacement (μm/min) b Coefficient of movement efficiency (CME) c
SFM 76.58 ± 2.05* 0.13 ± 0.003* 17.58 ± 1.50* 0.03 ± 0.002* 0.23 ± 0.02*
SFM + IGF 112.8 ± 3.6** 0.18 ± 0.005** 55.89 ± 3.87** 0.09 ± 0.005** 0.48 ± 0.02**
SFM + IGF + FF 80.5 ± 1.64 0.13 ± 0.002 31.07 ± 1.79** 0.05 ± 0.002** 0.39 ± 0.02**
FBS 105.04 ± 2.9 0.175 ± 0.005 67.30 ± 3.07 0.11 ± 0.005 0.64 ± 0.02
FBS + FF 59.61 ± 1.82* 0.01 ± 0.003* 16.83 ± 1.52* 0.02 ± 0.003* 0.27 ± 0.02*
FBS + NVP 94.47 ± 2.35 0.16 ± 0.005 45.27 ± 2.70* 0.07 ± 0.004* 0.47 ± 0.04*
FBS + FF + NAC 105.51 ± 2.6 0.17 ± 0.005 59.03 ± 2.50 0.09 ± 0.004 0.56 ± 0.02
  1. Individual tracks of LN-229 cell locomotion were generated by determination of cell centroid displacements with time-lapse images taken at 20 minutes intervals during a total observation period of 10 hours. The positions of the cell centroid were marked by an observer on a digitized image. Fifty cell tracks were recorded under each of the experimental conditions tested. The cell trajectories were presented in circular diagrams with the starting point of each trajectory situated in the diagram centre. a: the average speed of cell locomotion is defined as the total length of cell trajectory/time of recording (10 hrs). b: the average rate of cell displacement is defined as the total length of cell displacement from the starting point to the final cell position/time of recording (10 hrs). c: the ratio of cell displacement to cell trajectory length. CME would equal 1 for the cell moving persistently along one straight line in one direction and 0 for a random movement [68]. The values are given as the mean +/-SEM (n = 50). * Statistically different from FBS at p≤0.05. ** Statistically different from SFM at p≤0.05.