Genes differentially expressed in mutant and wild-type FLCN cell lines. (A) Heat-map representation of the genes differentially expressed in mutant and wild-type FLCN cell lines, and hierarchical clustering of the cell lines. Gene symbols are listed on the left side of each row. Three prominent groups of genes involved in signaling pathways were indicated on the left side of the gene symbols. (B) Deregulation of the key molecules in TGF-β signaling by FLCN knockdown in the UOK257-2 cells expressing wildtype FLCN. C, control cell line infected with empty retrovirus; KD, FLCN-knockdown cell line infected with a retrovirus expressing short hairpin RNA targeting FLCN.