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Figure 5 | Molecular Cancer

Figure 5

From: Tumor suppressor FLCN inhibits tumorigenesis of a FLCN-null renal cancer cell line and regulates expression of key molecules in TGF-β signaling

Figure 5

TGF-β1 induced SMAD3 phosphorylation and effects of AICAR, Compound C and rapamycin on TGF- β1 induction of TGFB2 and INHBA in FLCN-null and FLCN -expressing cells. (A) TGF-β1 induced SMAD3 phosphorylation in UOK257 and UOK257-2 cells. Cells were treated with 0, 0.1 and 1 ng/ml of TGF-β1 for 1 hr. (B) TGF-β1 induced TGFB2, INHBA and SMAD7 mRNA expression in UOK257 and UOK257-2 cells. Cell were treated with 1 ng/ml of TGF-β1 for 0, 6, 12 and 24 hr after serum starvation for 12 hr. (C) (a and b) Cells were serum-starved for 24 hr and treated with 0.5 mM AICAR (AI), 20 μM Compound C (CC) or 1 nM rapamycin (Rapa) for 12 hr. UOK257-2/vector, UOK257-2 cells infected with empty retrovirus; UOK257-2/FLCN-KD, UOK257-2 cells infected with retrovirus expressing shRNA targeting FLCN. (c and d) Cells were serum-starved for 15 hr and treated with 0.5 mM AICAR or 20 μM Compound C (CC) for 6 hr.

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Molecular Cancer

ISSN: 1476-4598