Figure 5

Analysis of EMT markers and E-cadherin expression on HT29 and RHT29 cells. (A1) Differential basal expression of some transcription factors related to the EMT process and (A2) markers of epithelial and mesenchymal phenotype were determined in the HT29 and RHT29 cell lines by qPCR. (B1) The effects of oxaliplatin in the gene expression of those transcription factors and (B2) EMT markers were also analyzed in cells treated with oxaliplatin 10 μM for 48 hours and in this case the results are shown as fold induction versus non-treated cells. Results represent the mean ± SEM of triplicates. Values that are significantly different between (A) groups or between (B) non-treated and treated groups by ANOVA's analysis are indicated by *p < 0.05, **p < 0.01, ***p < 0.001. C) HT29 and RHT29 cells were treated with oxaliplatin 10 μM for 48 hours and E-cadherin expression was detected by immunofluorescence and confocal microscope analysis. Scale bar is 50 μm.