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Figure 2 | Molecular Cancer

Figure 2

From: The Wnt inhibitory factor 1 restoration in prostate cancer cells was associated with reduced tumor growth, decreased capacity of cell migration and invasion and a reversal of epithelial to mesenchymal transition

Figure 2

Ectopic expression of WIF1 in PC3 cells inhibits AKT and GSK-3β, phosphorylation and LEF1/TCF transcriptional activity, leading to down-regulation of the expression of Wnt target genes: cyclin D1 and c-Myc. A, Western blot analysis of expression of WIF1, phospho-AKT, total AKT, Phospho-GSK-3β, total GSK-3β, cyclin D1 and c-Myc was shown by a representative blot from three independent experiments. B, WIF1 transfection reduced TCF4 transcriptional activity in PC3 cells compared with vector control-transfected cells, measured by the TOPFLASH reporter assay and adjusted by transfection efficiency.

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