Ectopic expression of WIF1 in PC3 cells results in a morphology change from a fibroblastic to an epithelial appearance, accompanied by up-regulating the expression of epithelial markers and down-regulating the expression of mesenchymal markers. A, representative photograph of transfected cells at 60% confluence was taken under an inverted phase-contrast light microscope at ×200 magnification. B, the transfectants were stained with anti-E-cadherin and Alex 488-conjugated secondary antibodies. Representative photographs were taken under a confocal microscope (Zeiss) at ×400 magnification. C, Western blot analysis of expression of E-cadherin, keratin-8, keratin-18, N-cadherin, vimentin, fibronectin, and β-actin shown by a representative blot from three independent experiments.