Ectopic expression of WIF1 in PC3 cells inhibits tumor growth in a xenograft model. PC3 cells (2 × 106) stably transfected with vector control or WIF1 were injected into the left flank of NCR-nu/nu (nude) mice. A, points, mean tumor volume (each group contains 13 mice); Bars, SD. B, at the termination of the study on day 48, tumors were excised from each mouse in different groups and weighed. Wet weight of tumors is represented as means of 13 tumors from individual mouse in each group. P< 0.05 at the termination of the experiments; Bars, ± SD. C, Immunohistochemical staining of ectopic E-cadherin, Keratin-18 and vimentin expression in harvested mouse tumor samples.