Expression levels of Noxa, Mcl-1, Bim and A1 in RCC lines treated with chemotherapeutic drugs.Cells from RCC cell lines 21, 26A, 30 and Caci-2 were treated for 24 h with the indicated drugs (100 nM vinblastine, 200 nM paclitaxel, 200 μM etoposide, 1 mM 5-FU). Total cell lysates were assayed for Noxa, Mcl-1 and Bim levels by Western blot analysis. Tubulin served as a loading control. The immunoblots shown here are representatives of three separate experiments. The mRNA-levels of A1 were measured by quantitative RT-PCR in cells that had been treated for 24 h with etoposide or not. The mean values of untreated controls were normalized to 100%. Data are mean/SEM from three separate experiments.