IGFBP7 and SLC25A36 expression in HPV-immortalised cells and cervical cancer cells transduced with hsa-miR-124 . A. Expression levels of IGFBP7, a potential target gene of hsa-miR-124, were increased in late passages of FK16B and FK18B cells, also showing increased methylation of hsa-miR-124, compared to their corresponding early passages. B. Effects of ectopic hsa-miR-124 expression on mRNA expression of IGFBP7 (grey bars) in SiHa and CaSki cells. Expression of SLC25A36 (white bars), a gene without an hsa-miR-124 target site, was also determined as a control. Expression in CaSki_ctrl and SiHa_ctrl was set to 100%, respectively. Results from 3 independent experiments showed that IGFBP7 expression was decreased in CaSki_miR-124 but not in SiHa_miR-124 cells compared to their parental and empty vector control cell lines. Expression of SLC25A36 was similar in cells with and without ectopic hsa-miR-124 expression.