Conditioned medium from Dasatinib-treated CAFs inhibits tumor cell proliferation. H1299 epithelial tumor cells were cultivated with conditioned medium (CM; plots 3, 4, and 5) or control medium (plots 1 and 2) for 48 hours before harvesting for BrdU labelling and propidium iodide staining. CM was collected from CAFs cultivated in the presence or absence of Dasatinib for 48 h in medium supplemented with 0.1% FCS. Control medium (0.1% FCS) with or without Dasatinib was collected following incubation for 48 h without cells. Upper panel: representative result; lower panel: absolute reduction of the percentage of BrdU positive tumor cells in S phase upon Dasatinib. Dasatinib was either added to control medium in the absence of cells (first bar; 2 minus 1), to CAF conditioned medium after collecting CM (second bar; 4 minus 3), or to CAFs for 48 hours before collecting CM (third bar; 5 minus 3). Values reflect means ± SEM from 3 independent experiments (statistics was performed with paired student's t-test, 2-tailed, Holm corrected; ***: p < 0.001).