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Figure 2 | Molecular Cancer

Figure 2

From: CXCR4/SDF1 mediate hypoxia induced chondrosarcoma cell invasion through ERK signaling and increased MMP1 expression

Figure 2

CXCR4 expression during normoxia and hypoxia in chondrosarcoma cells. A, CXCR4 mRNA expression in chondrosarcoma cell line JJ and normal chondrocytes (CH) in normoxia was analyzed by qRT-PCR. *, p < 0.02. B, CXCR4 mRNA expression in JJ cells in normoxia (N) and hypoxia (H) for 24 h (N1, H1) or 48 h (N2, H2) was quantitated by qRT-PCR. *, p < 0.05, compared to N1. #, p < 0.001, compared to N2. C, Representative electrophoresis of qRT-PCR products from B. Odd numbered lanes normoxia, even numbered lanes hypoxia; 24 and 48 h. Lanes 1-4: internal control (18S), lanes 5-8: Hif-1a, lanes 9-12: CXCR4. M is 50 bp molecular marker. D, Representative Western Blot of three independent experiments to detect CXCR4 and Hif-1a in JJ cells. Cell lysates were obtained after 48 h of culture in normoxia or hypoxia. Actin was used as loading control.

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