Figure 2From: CXCR4/SDF1 mediate hypoxia induced chondrosarcoma cell invasion through ERK signaling and increased MMP1 expressionCXCR4 expression during normoxia and hypoxia in chondrosarcoma cells. A, CXCR4 mRNA expression in chondrosarcoma cell line JJ and normal chondrocytes (CH) in normoxia was analyzed by qRT-PCR. *, p < 0.02. B, CXCR4 mRNA expression in JJ cells in normoxia (N) and hypoxia (H) for 24 h (N1, H1) or 48 h (N2, H2) was quantitated by qRT-PCR. *, p < 0.05, compared to N1. #, p < 0.001, compared to N2. C, Representative electrophoresis of qRT-PCR products from B. Odd numbered lanes normoxia, even numbered lanes hypoxia; 24 and 48 h. Lanes 1-4: internal control (18S), lanes 5-8: Hif-1a, lanes 9-12: CXCR4. M is 50 bp molecular marker. D, Representative Western Blot of three independent experiments to detect CXCR4 and Hif-1a in JJ cells. Cell lysates were obtained after 48 h of culture in normoxia or hypoxia. Actin was used as loading control.Back to article page