Figure 3

Hif-1a regulates CXCR4 expression. A and B, CXCR4 and HIF-1a expression after transfection with HIF-1a. JJ cells were transfected with HIF-1a expression construct or empty vector (EV). After 48 h, CXCR4 and HIF-1a expression was quantified by qRT-PCR; *, p < 0.02 and p < 0.01 respectively. C, D and E, CXCR4 and HIF-1a expression after HIF-1a knockdown. JJ cells were transfected with si-RNA directed against HIF-1a (HIF-1a si, lanes 2,4,6) or scramble siRNA (con-si, lanes 1,3,5) and CXCR4 and HIF-1a expression analyzed after 72 h of hypoxia with qRT-PCR (C, D) and Western blotting (E); *, p < 0.001. M is 50 bp molecular marker.