Figure 6

SDF1 increases MMP1 mRNA level and MMP1 activity during hypoxia. Tumor cells were cultured on matrigel with only medium (MOCK), SDF1 (10 ng/ml), AMD3100 0.1 μM, or SDF1+AMD3100 (AMD3100 was added 2 h before SDF1), or SDF1 + mmpi (MMPs inhibitor O-phenanthroline, 10 nM) for 24 h. Conditioned medium was then collected for ELISA and cells were harvested for RNA isolation. A, MMP-1 mRNA level was measured by qRT-PCR (*, p < 0.001, compared to Mock in hypoxia; #, p < 0.001, compared to SDF1 in hypoxia). B, active MMP-1 was measured in conditioned media by ELISA (*, p < 0.01, compared to Mock-H in hypoxia; **, p < 0.05, compared to SDF1 in hypoxia).