Figure 1

KCTD11 is down-regulated in several cancers. (A) Representative images of KCTD11 immunohistochemistry (IHC) of esophagus (a, b), lung (c, d), stomach (e, f), colon-rectum (g, h), urinary bladder (i, j) and thyroid (k, l) (40x magnification). Tissue arrays (Super Bio Chips; cat. n. MA, MAN, MB, MBN, MC, MCN; http://www.tissue-array.com/ver3/index.php) were incubated with 1 mg/ml affinity-purified rabbit polyclonal anti-KCTD11 antibody, as previously described [12]. (B) Graphic representation of KCTD11 IHC analysis of tissue arrays. Normal and cancer tissues were analyzed counting nuclear staining as percent point. 10 high power fields (hps 40x) were counted for each sample. Stromal and inflammatory cells were not selected for nuclear staining counting. The significance of differences between normal (N) and tumor (T) tissues means was estimated using Student's t-test. (* indicates p < 0.005; ** indicates p < 0.001). Larinx: N n = 5, T n = 7; Esophagus: N n = 9, T n = 10; Stomach: N n = 13, T n = 10; Colon-rectum: N n = 12, T n = 10; Urinary bladder: N n = 5, T n = 9; Lung: N n = 10, T n = 10; Breast: N n = 4, T n = 10; Gallbladder: N n = 8, T n = 6; Endometrium: N n = 7, T n = 10; Thyroid: N n = 10, T n = 10. n: number of cases for each group.