Figure 5From: Identification and characterization of retinoblastoma gene mutations disturbing apoptosis in human breast cancersSubcellular localization of pRb mutants. Immunofluorescence staining of RB1-deficient C-33 A cells transfected with the pcDNA3.1/V5-His-TOPO vector or one of the four plasmids RB1wild-type-V5, RB1Arg621Cys-V5, RB1Leu607Ile-V5, and RB1Arg698Trp-V5. All mutants display nuclear localization. Left: DAPI, Centre: Anti-V5 (Texas Red), Right: Overlay.Back to article page