Figure 1

Activity of dl 922-947 in immortalized and ovarian cancer cell lines. 1A: Cytotoxicity in MRC5 fibroblasts. MRC5 and MRC-VA cells were infected with dl 922-947 (MOI 0.01 - 10000 pfu/cell). Cell survival was assessed 144 hours later. 1B: Cytotoxicity and infectivity in ovarian cancer cells. Eight ovarian cancer cell lines were infected with dl 922-947 (MOI 0.001 - 1000 pfu/cell). Cell survival was assessed 120 hours later (left). Cell infectivity in TOV21G, IGROV1, A2780CP and SKOV3ip1 cells was assessed 24 h following infection with Ad-CMV-GFP (MOI 5 and 50 pfu/cell) using flow cytometry. Results are presented as percentage cells GFP positive (right). 1C: Viral protein expression and replication. Ovarian cancer cells were infected with dl 922-947 (MOI 10) and protein harvested up to 72 hours later. E1A expression was analyzed by immunoblot (left). TOV21G, IGROV1, A2780CP and SKOV3ip1 were infected with dl 922-947 (MOI 10). Viral replication was assessed up to 72 hours later by and TCID₅₀ assay. Horizontal lines represents input dose (right). 1D: Expression of Rb pathway components in ovarian cancer cells. Protein was harvested from asynchronous growing ovarian cancer cells, separated on SDS-PAGE cells and analyzed by immunoblot.