Figure 3

TSA blocks PMA-induced c-Jun but not c-Fos expression in HeLa cells. A and C. HeLa cells were pretreated with TSA (0-1 μM) for 1 h followed by treatment with PMA (50 ng/ml) for 2 h. Cell lysates (50 μg) containing equal amount of total proteins were analyzed by western blot using either anti-c-Jun or anti-c-Fos antibody. B and D. HeLa cells were pretreated with TSA and then with PMA under similar conditions as described above. Total RNA was isolated and the levels of c-jun and c-fos mRNAs were detected by semiquantitative RT-PCR. Actin was used as control.