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Figure 4 | Molecular Cancer

Figure 4

From: Transcriptional regulation of human osteopontin promoter by histone deacetylase inhibitor, trichostatin A in cervical cancer cells

Figure 4

TSA inhibits PMA-induced hyperacetylation of histones H3 and H4 and recruitment of RNA pol II and TFIIB to OPN promoter in HeLa cells. A-D. HeLa cells were pretreated with TSA for 1 h and then with PMA for 2 h. Cross-linked DNA-protein complexes were immunoprecipitated with anti-acetyl H3, anti-acetyl H4, anti-RNA pol II or anti-TFIIB antibody and PCR amplified using specific primers derived from the region of OPN promoter containing AP-1 binding site. For negative controls, normal mouse IgG was used. The data represents three experiments exhibiting similar results.

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Molecular Cancer

ISSN: 1476-4598