Figure 5

HDAC1 is involved in regulation of PMA-induced OPN promoter activity in HeLa cells. A. panels I-III. HeLa cells were transfected with either siRNAs to HDAC1, HDAC3 and HDAC4 or control siRNA. The expressions of various HDACs were analyzed by western blot using their specific antibodies. Actin served as loading control. B. HeLa cells were transfected with siRNAs to HDAC1, HDAC3 and HDAC4. These transfected cells were cotransfected with hOPN promoter (-500/+20) containing luciferase reporter gene along with Renilla luciferase, pRL vector and then treated with PMA. The luciferase activity was measured in cell lysates and normalized to Renilla luciferase activity. Fold-changes in luciferase activity with respect to control were calculated. Columns, mean of triplicate determinations; bar, S.D. *, p < 0.05.