Figure 6

Effect of TSA on cyclin D1 and uPA expression at protein and RNA levels in HeLa cells. A and C. HeLa cells were pretreated with TSA (0-1 μM) for 1 h followed by treatment with PMA (50 ng/ml) for 6 h. Cell lysates were analyzed by western blot using anti-cyclin D1 or anti-uPA antibody. B and D. Total RNA was isolated from HeLa cells treated under similar conditions and cyclin D1 and uPA mRNA levels were detected by semi-quantitative RT-PCR. Actin was used as loading control for both western blot and RT-PCR.