Figure 7
TSA suppresses cervical tumor growth in response to PMA in NOD/SCID mice model. A. HeLa cells (1 × 106/0.2 ml) were mixed with matrigel (1:1 ratio) and injected subcutaneously into the flanks of mice. Six mice were kept in each group. Either PMA alone or in combination with two doses of TSA was injected intratumorally. After 4 weeks, mice were sacrificed and tumors were excised. The tumor volumes were calculated and the growth kinetics in tumor volume in fold change vs time in weeks is represented in the form of graph. The error bars represent the standard error of mean (SEM). *, p < 0.05. B and C. Tumor lysates were analyzed by western blot using anti-OPN, anti-c-Jun, anti-cyclin D1 and anti-uPA antibodies. Tumors were also analyzed by semiquantitative RT-PCR using gene specific primers. Actin was used as control. D. Schematic representation of PMA-induced OPN transcription through c-Jun leading to enhanced cervical tumor growth and TSA suppresses this process.