Figure 10

E-cadherin positive metastatic foci originate from E-cadherin negative primary tumors. A) Left, human MDA-MB-231 breast cancer cell xenograft in a mouse inguinal fat pad (H&E); middle, human-specific E-cadherin antibody indicates the absence of E-cadherin expression in the center of the primary tumor; right, absence of human-specific E-cadherin labeling at the periphery of the tumor. B) Micrometastases in the lung originating from the primary xenograft in A. Immunoperoxidase labeling of diseased portions of the mouse lung indicate the presence of human E-cadherin-positive MDA-MB-231 cancer cells; bottom adjacent.