E-cadherin expression alters cell morphology. A) Cell morphology as examined by phase contrast microscopy (left column) and E-cadherin expression (red) as detected by immunofluorescence (right column) B) Immunblot analysis illustrates ectopic expression of E-cadherin in 231-Ecad cells as well as expression of various epithelial and mesenchymal markers in the E-cadherin mutants. C) Immunofluorescence of vimentin, cytokeratin-18 and actin cytoskeleton (rhodamine phalloidin). Shown are representative of at least three different assessments using one of two independent clones of each cell variant. D) Quantification of fluorescence using ImageJ, n = 20 cells, p < 0.05.