Figure 2From: The potassium channel Ether à go-go is a novel prognostic factor with functional relevance in acute myeloid leukemiaExpression of hEag1 in leukemia cell lines and primary AML cells. A: hEag1 mRNA levels determined by real-time PCR, expressed as relative to that in UT-7 cells. B: hEag1 protein content in cell lines and primary cells from hEag1 positive patients measured by flow cytometry. The background rightward shift induced by anti-hEag1 antibody mAb49 in the PCR-negative P2 cells was set as zero value. C: hEag1 siRNA treatment (lower panels) strongly diminished the fluorescence shift attributable to hEag1 expression in PLB-985 and K562 cells. The gray peak indicates control secondary fluorescent antibody staining.Back to article page