Figure 6

Cell cycle distribution and apoptosis in the presence of hEag1 inhibitors in vitro. A. The indicated cell lines were incubated with 4 μM astemizole (AST), 20 μM imipramine (IMI) or 10 μg/mL anti-hEag1 mAb56 for 2 days. Cell cycle phases were measured after staining with propidium iodide by flow cytometry. Only mAb56 increased significantly the proportion of cells in the S phase in K562 and HEL cells. B. K-562 and primary P4 cells were incubated with 4 μM astemizole or mAb56 antibody for 2 days, respectively and apoptosis was measured by flow cytometry. A clear increase in Annexin V fluorescence (apoptosis) was observed in both determinations.