Figure 2
From: Characterizing cancer cells with cancer stem cell-like features in 293T human embryonic kidney cells
293T cells form spheres in suspension culture that exhibit cancer stem cell phenotype. A, B) Sphere formation in 293T cells seeded in self renewal promoting suspension culture conditions. Passage of 293T spheres gives rise to higher proportion of secondary spheres. C) Flow cytometric analysis of expression of CD24 and CD44 in 293T cells. Cells were cultured under adherent (2D) or tumor-initiating cells enrichment condition (3D). There is an increase of CD44+/CD24- population in cells cultured in 3D vs. 2D culture conditions. D) Flow cytometric analysis of ALDH activity showed that the 3D culture condition enriches for ALDH-positive cells compared to the 2D culture of 293T cells.