NF-κB inhibitor parthenolide blocks 2ME2 and Doc-mediated apoptosis in LNCaP cells. DAPI apoptosis analysis (% condensed fragmented nuclei) showing that parthenolide (P; 10 μM) blocks 2ME2 (M; 5 μM) and Doc (D; 1 nM) apoptosis in LNCaP (72 h) cells. n = 7, four independent experiments; *, P < 6 × 10-4. Western blot analysis shows that P inhibits the M and D increase in cleaved PARP (72 h), P-IκBα/IκBα (24 h), P-p65/p65 (24 h), p53 (72 h), and the M/D decrease in XIAP (72 h). Coomassie blue stain of total protein is loading control.