NF-κB activator BA stimulates 2ME2 and Doc-mediated apoptosis in LN-AI and DU145 cells. DAPI apoptosis assay (% condensed fragmented nuclei) showing that BA (B; 10 μM) increases 2ME2 (M; 5 μM) and Doc (D; 1 nM) apoptosis (MB and DB) in LN-AI (24 h) and DU145 (72 h) compared to M, B, and control cells. n = 6-8, three to four independent experiments; *, P < 0.004. Western blot analysis shows greater cleaved PARP in MB and DB compared to M, D, or B alone in LN-AI but not in DU145 cells. MB and DB increases P-IκBα/IκBα and P-p65/p65 in LN-AI and DU145 cells at 24 h compared to M, D, B, and control cells, suggesting activation of NF-κB. MB and DB decreases IκBα, p53 and XIAP compared to M and D alone; B alone also decrease IκBα, p53, and XIAP compared to control cells. Coomassie blue stain of total protein is loading control.