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Figure 1 | Molecular Cancer

Figure 1

From: Cadmium down-regulates expression of XIAP at the post-transcriptional level in prostate cancer cells through an NF-κB-independent, proteasome-mediated mechanism

Figure 1

Effect of cadmium on XIAP protein and mRNA levels in prostate cancer cells. (A) PC-3 and DU-145 cells were treated with indicated concentrations of cadmium for 16 hours. Levels of XIAP, cIAP1 and c IAP2 proteins were detected in cell lysates by immunoblotting using specific antibodies. Expression of β-actin was used to control equal protein loading. Representative data from one of four experiments is shown. (B) The effect of cadmium on the viability of PC-3 and DU-145 cells. Cells were cultured in the absence or presence of cadmium (30 μM) for 16 hours, harvested and stained with propidium iodide followed by flow cytometry analysis. X axis represents fluorescence intensity, Y axis represents cell number. Representative data from one of three experiments. (C) PC-3 and DU-145 cells were treated with cadmium (30 μM) for indicated periods of time. XIAP mRNA levels were examined by real-time PCR analysis as described in Materials and Methods.

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Molecular Cancer

ISSN: 1476-4598