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Figure 5 | Molecular Cancer

Figure 5

From: Cadmium down-regulates expression of XIAP at the post-transcriptional level in prostate cancer cells through an NF-κB-independent, proteasome-mediated mechanism

Figure 5

Cadmium sensitizes prostate cancer cells to TNF-alpha-mediated apoptosis. (A) PC-3 cells were pre-treated with cadmium (30 μM) for 16 hours followed by stimulation with TNF-α (20 ng/ml) with or without Z-VAD.FMK (50 μM) for an additional 20 hours. The percentage of apoptotic cells was determined by the APO-BRDU assay followed by flow cytometry analysis. Representative data from one of three experiments is shown. (B) PC-3 cells were pre-incubated with or without Z-VAD.FMK (50 μM) for 30 minutes followed by treatment with cadmium (30 μM) for 16 hours. XIAP expression was detected in cell lysates by immunoblotting with specific antibody. (C) PC-3 cells were cultured in medium alone or in the presence of cadmium (30 μM) for 7 days followed by treatment with indicated concentrations of cadmium for 16 hours. XIAP expression was detected in cell lysates by immunoblotting. (D) Aliquots of cells described in panel C were pre-treated with cadmium (30 μM) for 16 hours followed by stimulation with TNF-α (20 ng/ml) for an additional 20 hours. The percentage of apoptotic cells was determined by the APO-BRDU assay. Representative data from one of three experiments is shown.

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