Exon array analyses of alternative splicing of Dmbt1 gene with FIRMA. A, FIRMA scores calculated from array data for all the mice are plotted in the top graphic. Each line represents values from a single mouse. Highlighted in colour are 8-week old mice [black, WT on control diet; green, PTEN null on control diet; blue, PTEN null on LSF diet; red, PTEN null on HSF diet]. B, The normalised logged intensities of each probeset are plotted (note that certain exons are represented by more than one probeset). The blue connecting lines map these probesets to (C) gene models as defined by Affymetrix (green) and Ensembl (orange). Transcript isoforms known for this gene are plotted in dark blue. The longer isoform is designated in Ensembl as Dmbt1-202 and the shorter as Dmbt1-201 (see Figure 13). The five regions highlighted in the plot show exons with statistically significant difference in FIRMA scores between 8-week PTEN null mice on HSF diet and WT mice on control diet.