DNA Methylation analysis of TES promoter in GC. TES mRNA (A) and protein (B) expression were significantly down regulated in three GC cell lines (MGC803, HGC27 and SGC7901). Methylation analysis of TES promoter in three GC cell lines (C) and in 10 pairs of GC samples (D). Complete methylation was shown in MGC803 and HGC27 cells and in most GC samples. After DAC treatment, the complete methylation of TES promoter changed to partial methylation and nonmethylation in MGC803 and HGC27 cells, respectively (E). DAC treatment also reversed TES mRNA (F) and protein (G) expression in MGC803 and HGC27 cells. "M", "U" and"M-P"represent methylated CPG sequences, unmethylated CPG sequences and a positive control for methylation, respectively. "-" and "+" represent before and after DAC treatment, respectively.