Effect of the c-jun siRNA on Mcl-1 expression level. (A) After transfection and incubation, total RNA has been extracted and retro-transcribed in cDNA. A real time PCR has been performed with specific primers for Mcl-1 and for RPL13A, a house-keeping gene. Results are expressed in induction level by comparison with the reference condition, normoxia. (B) Mcl-1 was detected in total cell extracts by western blotting, using specific antibody. α-tubulin was used to assess the total amount of proteins loaded on the gel.