Figure 3

Cytotoxic effects of SB-T-1214 on colon CSC-enriched cell populations grown on type I collagen-coated surfaces. FACS sorted CD133^high/CD44^high cells derived from colon cancer cell lines (HCT116 is shown) and peritoneal fluid of patient with metastatic colon cancer were plated for a short time on collagen type I-coated plates in the MSCB medium. Although a majority of these cells were killed after 48 hours incubation with 100 nM of SB-T-1214, some percentage of the cells survived treatment (A-C, D-F). The cells which survived treatment expressed multiple abnormalities, including greatly enlarged size (G-I, K), multiple nuclei (E, G-I), significant increase in the number of long (J), and knobby (F, I) projections and severe vacuolization (K). A-C: magnification 10×; D-K: magnification 40×.