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Figure 4 | Molecular Cancer

Figure 4

From: New-generation taxoid SB-T-1214 inhibits stem cell-related gene expression in 3D cancer spheroids induced by purified colon tumor-initiating cells

Figure 4

Cytotoxic effects of the SB-T-1214 against colonospheres grown in 3D cultures. Ten day-old colonospheres grown from FACS sorted CD133high/CD44high HCT116 cells on the ultra-low attachment 6-well plates (Corning) were treated with 100 nM SB-T-1214 for 48 hours, then drug-containing medium was replaced with regular MSCBM and analyzed after 24 hours. Treatment with drug induced a profound loss of sphere integrity (A, C) and apoptosis in the majority of the sphere cells (D-E; FACS analysis with the annexin V-FITC and propidium iodide). The FITC-conjugated drug showed efficient penetration into spheroid after 30 min of incubation (B). To evaluate possible alterations in sphere-forming capacity, control and drug treated spheroids/cells were dissociated to a single cell suspension and an equal number of viable cells (400 cells) were plated on ULA 6-well plates in MSCB medium containing 10% Matrigel. The number of secondary spheroids was counted ten days after plating (F).

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