Figure 2

Gene expression differences of Trp53^ΔEC and Rb^ΔEC; Trp53^ΔEC mouse tumors based on histological grade. Gene expression deregulation related to carcinoma differentiation was done using supervised Ttest analysis between poorly differentiated carcinomas and undifferentiated/spindle carcinomas. Results are shown for 2% of probesets overexpressed (n = 902, FDR corrected p-val = 0.002) or underexpressed (n = 902, FDR corrected p-val = 0.001) in undifferentiated/spindle carcinomas. (a) Hierarchical clustering analysis using euclidean distance and average linkage clustering of probesets and all carcinomas: poorly differentiated (green), undifferentiated/spindle (red), and mixed, containing differentiated and undifferentiated areas (blue). (b) Enrichment analysis in GO biological processes of the deregulated genes evidenced overexpression of genes involved in vasculature development, cell adhesion, and endocytosis (red bars), and underexpression of genes involved in keratinocyte differentiation or cell death (green bars) in undifferentiated/spindle carcinomas. p-val: significance of enrichment.