Brightest point projection images (BPI) of tumor growth patterns. The same tumors shown in Figure 3 were analyzed using BPI to visualize global differences in tumor growth patterns. Control T98G and T98G TWIST1 over-expressing tumor images are generated from total of 140 and 164 optical sections, respectively, collected by confocal microscope using ImageJ software. Control tumors possess a more cohesive pattern and localized growth pattern while T98G TW tumors demonstrate a markedly diffuse pattern of growth (type 3 invasive growth pattern). Arrows indicate regions of diffuse T98G TW tumor cell outgrowth.